Genetic Mapping of Grain Shape Associated QTL Utilizing Recombinant Inbred Sister Lines in High Yielding Rice (Oryza sativa L.)
نویسندگان
چکیده
Grain shape is a key factor for yield and quality in rice. To investigate the genetic basis of grain high-yielding hybrid rice variety Nei2You No.6, set recombinant inbred sister lines (RISLs) were used to map quantitative trait loci (QTLs) determining length (GL), width (GW), length-width ratio (LWR) four environments. A total 91 medium/minor-effect QTL detected using high-density consisting 3203 Bin markers composed single nucleotide polymorphisms, among which 64 formed 15 clusters. Twelve clusters co-localized with previously reported shape/weight. Three new detected: qGL-7a, qGL-8, qGL-11a. cluster, qLWR-12c/qGW-12, was across all environments phenotypic variation explained (PVE) ranging from 3.67% 11.93%, subsequently validated paired F17 progeny tightly linked marker assay F10 generation. Subsequently, 17 candidate genes qLWR-12c/qGW-12 431 Kb interval utilizing bulk segregant analysis (BSA). Among these, OsR498G1222170400, OsR498G1222171900, OsR498G1222185100, OsR498G1222173400, OsR498G1222170500 best candidates, lays foundation further cloning will facilitate high-yield breeding
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ژورنال
عنوان ژورنال: Agronomy
سال: 2021
ISSN: ['2156-3276', '0065-4663']
DOI: https://doi.org/10.3390/agronomy11040705